Mutation detail:
| Mutation site | 28076C>A |
| Virus | SARS-CoV-2 |
Mutation level  |
Nucleotide level |
| Gene/protein/region type | ORF8 |
| Gene ID | 43740577 |
| Country | - |
| Mutation type |
- |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_045512 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF8 protein |
| Uniprot protein ID | P0DTC8 |
| Protein length | 121 amino acids |
| Protein description | The SARS-CoV-2 ORF8 gene spans 366 nucleotides (nt), is located between position 27,894 and 28,259 of the virus genome, following the ORF7b and preceding the N genes, respectively. The ORF8 gene is part of a hypervariable genomic region of - 430 bp in length that has been recognized as a recombination hotspot, also highly susceptible to deletions and nt substitutions. ORF8 protein comprehends an N-terminal signal peptide for transmembrane (TM) import and secretion, an internal -sandwich core and a C-terminal TM region followed by a stretch of basic residues. |
Literature information:
| Pubmed ID | 33137303 |
| Clinical information | No |
| Disease | - |
| Published year | 2020 |
| Journal | JOURNAL OF IMMUNOLOGICAL METHODS |
| Title | SARS-CoV-2 variants lacking a functional ORF8 may reduce accuracy of serological testing |
| Author | Filipe Pereira |
| Evidence | I recently identified several nonsense mutations and additional deletions in the ORF8 gene that either remove or significantly change the ORF8 protein (Pereira, 2020). Currently, 17 nonsense mutations and eight deletions resulting in truncated ORF8 proteins, or even with the complete removal of the gene, are described in public databases (Table 1 ). |