Mutation detail:
| Mutation site | K660R |
| Virus | Influenzavirus A H1N1 |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | PB2 |
| Gene ID | 23308131 |
| Country | Netherlands |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | FJ966079.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Polymerase PB2 |
| Uniprot protein ID | C3W5X5 |
| Protein length | 759 amino acids |
| Protein description | PB2 plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. |
Literature information:
| Pubmed ID | 24699508 |
| Clinical information | No |
| Disease | - |
| Published year | 2014 |
| Journal | PLoS One |
| Title | Mass spectrometry-based comparative sequence analysis for the genetic monitoring of influenza A(H1N1)pdm16 virus |
| Author | Jairo Gooskens,Jessika C Zevenhoven-Dobbe,Eric C Claas,Aloys C M Kroes,Clara C Posthuma |
| Evidence | The majority of SNPs (246/456) resulted in silent mutations and few SNPs (210/456) encoded for non-relevant amino acid substitutions in NA gene (V106I (n=61), V203M (n=1), N248D (n=62), S286G (n=1)), PB1-F2 gene (T34A (n=1), V113A(n=1)), PB2 gene (K660R (n=2)) and NS1 gene segments (I123V (n=61), N133D (n=16), S135N (n=1), G154R (n=1), V194I (n=1), D207N (n=1)). The degree of discordance was limited (31/487 SNPs) and only 21 amino acid substitutions differed among the results generated by MSCSA and Sanger sequencing. |