Mutation detail:
Mutation site | K660R |
Virus | Influenzavirus A H1N1 |
Mutation level ![]() |
Amino acid Level |
Gene/protein/region type | PB2 |
Gene ID | 23308131 |
Country | Netherlands |
Mutation type ![]() |
nonsynonymous mutation |
Genotype/subtype/clade | - |
Sample ![]() |
Human |
Variants | - |
Viral reference sequence | FJ966079.1 |
Drug/antibody/vaccine | - |
Transmissibility ![]() |
- |
Transmission mechanism | - |
Pathogenicity ![]() |
- |
Pathogenicity mechanism | - |
Immune escape mutation | - |
Immune escape mechanism | - |
RT-PCR primers probes | - |
Protein detail:
Protein name | Polymerase PB2 |
Uniprot protein ID | C3W5X5 |
Protein length | 759 amino acids |
Protein description | PB2 plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. |
Literature information:
Pubmed ID | 24699508 |
Clinical information | No |
Disease | - |
Published year | 2014 |
Journal | PLoS One |
Title | Mass spectrometry-based comparative sequence analysis for the genetic monitoring of influenza A(H1N1)pdm16 virus |
Author | Jairo Gooskens,Jessika C Zevenhoven-Dobbe,Eric C Claas,Aloys C M Kroes,Clara C Posthuma |
Evidence | The majority of SNPs (246/456) resulted in silent mutations and few SNPs (210/456) encoded for non-relevant amino acid substitutions in NA gene (V106I (n=61), V203M (n=1), N248D (n=62), S286G (n=1)), PB1-F2 gene (T34A (n=1), V113A(n=1)), PB2 gene (K660R (n=2)) and NS1 gene segments (I123V (n=61), N133D (n=16), S135N (n=1), G154R (n=1), V194I (n=1), D207N (n=1)). The degree of discordance was limited (31/487 SNPs) and only 21 amino acid substitutions differed among the results generated by MSCSA and Sanger sequencing. |