Mutation detail:
| Mutation site | N133D |
| Virus | Influenzavirus A H1N1 |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | NS1 |
| Gene ID | 23308111 |
| Country | Netherlands |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | FJ966086.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Nonstructural Protein 1 |
| Uniprot protein ID | C3W611 |
| Protein length | 219 amino acids |
| Protein description | The non-structural protein 1 (NS1) is a crucial influenza A virus immune regulator that antagonizes antiviral response. The NS1 protein counteracts the production of IFNs and the activities of IFN-induced proteins that restrict influenza virus replication. Furthermore, NS1 is a multifunctional regulatory viral protein that plays a critical role as a posttranscriptional regulatory factor in the life cycle of influenza virus; specifically, binding virion RNA. NS1 protein consists of 2 distinct functional domains connected by a flexible linker region (LR): an N-terminal RNA- binding domain (RBD, aa 1-73) and a C-terminal effector domain (ED, aa 74-207). The nucleotide sequence (NLS) (aa 35-41) overlaps the sequences needed for dsRNA binding. |
Literature information:
| Pubmed ID | 24699508 |
| Clinical information | No |
| Disease | - |
| Published year | 2014 |
| Journal | PLoS One |
| Title | Mass spectrometry-based comparative sequence analysis for the genetic monitoring of influenza A(H1N1)pdm18 virus |
| Author | Jairo Gooskens,Jessika C Zevenhoven-Dobbe,Eric C Claas,Aloys C M Kroes,Clara C Posthuma |
| Evidence | The majority of SNPs (246/456) resulted in silent mutations and few SNPs (210/456) encoded for non-relevant amino acid substitutions in NA gene (V106I (n=61), V203M (n=1), N248D (n=62), S286G (n=1)), PB1-F2 gene (T34A (n=1), V113A(n=1)), PB2 gene (K660R (n=2)) and NS1 gene segments (I123V (n=61), N133D (n=16), S135N (n=1), G154R (n=1), V194I (n=1), D207N (n=1)). The degree of discordance was limited (31/487 SNPs) and only 21 amino acid substitutions differed among the results generated by MSCSA and Sanger sequencing. |