AVM

Protein name	Polymerase PB2
Uniprot protein ID	C3W5X5
Protein length	759 amino acids
Protein description	PB2 plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex.

Pubmed ID	25122786
Clinical information	No
Disease	-
Published year	2014
Journal	JOURNAL OF VIROLOGY
Title	The Temperature-Sensitive and Attenuation Phenotypes Conferred by Mutations in the Influenza Virus PB2, PB1, and NP Genes Are Influenced by the Species of Origin of the PB2 Gene in Reassortant Viruses Derived from Influenza A/California/07/2009 and A/WSN/
Author	Andrew J. Broadbent,Celia P. Santos,Rachel A. Godbout,Kanta Subbarao
Evidence	The following mutations were made in the WSN, CA07, and VN04 plasmids: PB1-K391E, -E581G, and -A661T and PB2-N265S. In addition, a D34G mutation was made in the WSN NP plasmid and an S34G mutation was made in the VN04 NP plasmid. The CA07 NP plasmid already contained a glycine at amino acid position 34, so it was not necessary to mutate the plasmid.