AVM

Protein name	Nucleocapsid Protein
Uniprot protein ID	C3W621
Protein length	498 amino acids
Protein description	NP encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Pubmed ID	25122786
Clinical information	No
Disease	-
Published year	2014
Journal	JOURNAL OF VIROLOGY
Title	The Temperature-Sensitive and Attenuation Phenotypes Conferred by Mutations in the Influenza Virus PB2, PB1, and NP Genes Are Influenced by the Species of Origin of the PB2 Gene in Reassortant Viruses Derived from Influenza A/California/07/2009 and A/WSN/
Author	Andrew J. Broadbent,Celia P. Santos,Rachel A. Godbout,Kanta Subbarao
Evidence	The following mutations were made in the WSN, CA07, and VN04 plasmids: PB1-K391E, -E581G, and -A661T and PB2-N265S. In addition, a D34G mutation was made in the WSN NP plasmid and an S34G mutation was made in the VN04 NP plasmid. The CA07 NP plasmid already contained a glycine at amino acid position 34, so it was not necessary to mutate the plasmid.