Mutation detail:
| Mutation site | R84S |
| Virus | Human rhinovirus |
Mutation level  |
Amino acid level |
| Gene/protein/region type | RdRp |
| Gene ID | 1493933 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | A |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | X02316.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | RNA-directed RNA polymerase |
| Uniprot protein ID | P07210 |
| Protein length | 460 amino acids |
| Protein description | Replicates the viral genomic RNA on the surface of intracellular membranes. RdRp may form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss+RNA genomes are either translated, replicated or encapsidated. |
Literature information:
| Pubmed ID | 11602738 |
| Clinical information | No |
| Disease | - |
| Published year | 2001 |
| Journal | Journal Of Virology |
| Title | Biochemical and genetic studies of the initiation of human rhinovirus 2 RNA replication: identification of a cis-replicating element in the coding sequence of 3A(pro) |
| Author | K Gerber,E Wimmer,A V Paul |
| Evidence | Mutation R84S was introduced into pProEX HTb/3CDpro(3CproC147A) by site-directed mutagenesis using primers |