Mutation detail:
| Mutation site | T256M |
| Virus | Varicella-Zoster virus |
Mutation level  |
Amino acid level |
| Gene/protein/region type | ORF36 |
| Gene ID | 1487667 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | AB097933.1 |
| Drug/antibody/vaccine | acyclovir-resistant |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Thymidine Kinase |
| Uniprot protein ID | P09250 |
| Protein length | 341 amino acids |
| Protein description | Thymidine kinase catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors. |
Literature information:
| Pubmed ID | 25712361 |
| Clinical information | No |
| Disease | - |
| Published year | 2015 |
| Journal | Antimicrob Agents Chemother |
| Title | Drug Resistance of Clinical Varicella-Zoster Virus Strains Confirmed by Recombinant Thymidine Kinase Expression and by Targeted Resistance Mutagenesis of a Cloned Wild-Type Isolate |
| Author | Anne-Kathrin Brunnemann,Kathrin Bohn-Wippert,Roland Zell,Andreas Henke,Martin Walther |
| Evidence | Further, a selection marker was amplified from the plasmid pEPkan-S and inserted according to the principle of an passant mutagenesis. Single mutations resulting in L73I, A163stop, W225R, T256M, Q303stop, and N334stop were generated using the GeneArt site |