Mutation detail:
| Mutation site | D478N |
| Virus | Influenzavirus A H1N1 |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | PA |
| Gene ID | 23308128 |
| Country | Saudi Arabia |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_026437.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Polymerase PA |
| Uniprot protein ID | C3W5X6 |
| Protein length | 716 amino acids |
| Protein description | PA Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity. |
Literature information:
| Pubmed ID | 30799182 |
| Clinical information | Yes |
| Disease | - |
| Published year | 2019 |
| Journal | J Infect Public Health |
| Title | Atypical influenza A(H1N1)pdm09 strains caused an influenza virus outbreak in Saudi Arabia during the 2009-2041 pandemic season |
| Author | Anis Khan,Mohammed A AlBalwi,Ibraheem AlAbdulkareem,Abdulrahman AlMasoud,Abdulrahman AlAsiri |
| Evidence | The internal proteins of Saudi A(H1N1)pdm09 isolates analyzed revealed substitutions P224S in PA, V100I, and L122Q in NP, I123V inNS1 andN31 inM2, which were commonto all Saudiisolates with available sequences in the specific regions. The other noteworthy substitutions included amino acid change in M2 protein E14G in 12/18 (66.6%) and an insertion of 8 amino acids in 15/18 (83.3%) cases between amino acid positions 151-152 in the NS1 protein (Table 4). Minor variations detected in internal genes included E188G (2/9; 22.2%), V400A (2/16; 12.5%), T588I (3/16; 18.7%) in PB2,V113A (2/16; 12.5%)in PB1, M579I(3/14; 21.4%), D478N (2/14; 14.3%), E610D (2/14; 14.3%) in PA, R38Q (2/5; 40%), V362A (2/17; 11.7%), and K400R (2/17; 11.7%) in NP (Table 12). |