Mutation detail:
| Mutation site | E1269D |
| Virus | Human respiratory syncytial virus |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | L |
| Gene ID | 1494467 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | A |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | M11486.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Polymerase |
| Uniprot protein ID | P28887 |
| Protein length | 2165 amino acids |
| Protein description | The core polymerase in RSV is composed of two proteins, a 250 kDa polymerase subunit (L), which contains the RNA-dependent RNA polymerase, the polyribonucleotidyl transferase (PRNTase, capping), and the methyltransferase enzymatic domains essential for viral transcription and replication, and the 27 kDa phosphoprotein (P) accessory protein. . The L protein caps mRNA by a unique RNA-GDP polyribonucleotidyl transferase activity mapped to conserved region V (CRV). Methylation of the cap at the guanine-N-7 and ribose-2-O positions is catalyzed by a unique dual specificity methyltransferase activity that has been functionally mapped to region VI of the L protein. |
Literature information:
| Pubmed ID | 16189012 |
| Clinical information | No |
| Disease | - |
| Published year | 2005 |
| Journal | Journal of virology |
| Title | Inhibitors of Respiratory Syncytial Virus Replication Target Cotranscriptional mRNA Guanylylation by Viral RNA-Dependent RNA Polymerase |
| Author | Michel Liuzzi,Stephen W. Mason,Mireille Cartier,Carol Lawetz,Robert S. McCollum |
| Evidence | Each of the three resistant strains harbored a different single nucleotide substitution in the L gene, resulting in one of the following amino acid changes: I1381S, E1269D, or L1421F (Fig.3A). No mutations in the other RNP subunit genes from the inhibitor |