Mutation detail:
| Mutation site | D701N |
| Virus | Influenzavirus A H1N1 |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | PB2 |
| Gene ID | 23308131 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | FJ966079.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Polymerase PB2 |
| Uniprot protein ID | C3W5X5 |
| Protein length | 759 amino acids |
| Protein description | PB2 plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. |
Literature information:
| Pubmed ID | 21325408 |
| Clinical information | No |
| Disease | - |
| Published year | 2011 |
| Journal | JOURNAL OF VIROLOGY |
| Title | Impact of Amino Acid Mutations in PB2, PB1-F2, and NS1 on the Replication and Pathogenicity of Pandemic (H1N1) 2009 Influenza Viruses |
| Author | Makoto Ozawa,Sarmila Basnet,Lisa M. Burley,Gabriele Neumann,Masato Hatta |
| Evidence | We evaluated the effect of this mutation with or without an NS1 mutation that exhibits increased pathogenicity in mice (NS1-W-RSKV) and one of the following two PB2 mutations: a glutamic acid-to-lysine change at position 627 (PB2-E627K) or an aspartic acid-to-asparagine substitution at position 701 (PB2-D701N). |