Mutation detail:
| Mutation site | R502C |
| Virus | SARS-CoV-2 |
Mutation level  |
Amino acid level |
| Gene/protein/region type | ORF1ab(helicase) |
| Gene ID | 43740578 |
| Country | Oman |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | GR |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_045512.2 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF1ab polyprotein |
| Uniprot protein ID | P0DTC1 |
| Protein length | 7096 amino acids |
| Protein description | ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies. |
Literature information:
| Pubmed ID | 33359061 |
| Clinical information | No |
| Disease | - |
| Published year | 2021 |
| Journal | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES |
| Title | Molecular epidemiology of COVID-19 in Oman: A molecular and surveillance study for the early transmission of COVID-19 in the country |
| Author | Samira Al-Mahruqi, Adil Al-Wahaibi, Abdul Latif Khan, Amina Al-Jardani, Sajjad Asaf |
| Evidence | Two unique missense mutations were detected in this study; I280V in the NSP15 and R502C in the NSP13, which existed at low frequencies of 6 (5.3%) and 2 (2.1%), respectively (Supplementary Figure S1). The mutation I280V belonged to lineage B.1.113 (GH), it was detected in cluster V in Al Batinah South and Muscat. The second mutation was detected in two cases that belonged to B.1.1 (GR) lineage within the E cluster of the Al Batinah South region (EPI_ISL_491143 and EPI_ISL_491144) as shown in Figure 2 . |