Mutation detail:
| Mutation site | T442I |
| Virus | SARS-CoV-2 |
Mutation level  |
Amino acid level |
| Gene/protein/region type | ORF1ab(RdRp) |
| Gene ID | 43740578 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_045512 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF1ab polyprotein |
| Uniprot protein ID | P0DTC1 |
| Protein length | 7096 amino acids |
| Protein description | ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies. |
Literature information:
| Pubmed ID | 33677109 |
| Clinical information | No |
| Disease | - |
| Published year | 2021 |
| Journal | INFECTION GENETICS AND EVOLUTION |
| Title | The extent of molecular variation in novel SARS-CoV-2 after the six-month global spread |
| Author | Ngoc-Niem Bui,Yu-Tzu Lin,Su-Hua Huang,Cheng-Wen Lin |
| Evidence | The phylogenetic analysis of genome sequences from 4230 SARS-CoV-2 variants by the NJ method indicated four haplotypes with the unique nucleotide variation and amino acid changes: T27879C (ORF8 L84S) in clade 1 (1072 variants, 25.34%), A23138G (spike D614G) in clade 2 (2688 variants, 63.54%), G10818T (nsp6 L37F), C14540T (nsp12 T442I), and G25879T (ORF3a V251F) in clade 3 (109 variants, 2.58%), and miscellaneous changes in clade 4 (361 variants, 8.54%) (Fig. 2A, Table 1). |