Mutation detail:
| Mutation site | 18194G>T |
| Virus | SARS-CoV-2 |
Mutation level  |
Nucleotide level |
| Gene/protein/region type | ORF1ab(3'-to-5' exonuclease) |
| Gene ID | 43740578 |
| Country | Iran |
| Mutation type |
- |
| Genotype/subtype/clade | 20G |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_045512 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF1ab polyprotein |
| Uniprot protein ID | P0DTC1 |
| Protein length | 7096 amino acids |
| Protein description | ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies. |
Literature information:
| Pubmed ID | 33836204 |
| Clinical information | No |
| Disease | - |
| Published year | 2021 |
| Journal | VIRUS RESEARCH |
| Title | SARS-CoV-2 presented moderately during two episodes of the infection with lack of antibody responses |
| Author | Mostafa Salehi-Vaziri, Mir Davood Omrani, Mohammad Hassan Pouriayevali, Fatemeh Fotouhi, Mohammad Banifazl |
| Evidence | Genomic sequence analysis of the first virus identified 11 mutated positions compared to the reference genome. The second virus, had six additional mutations from which 10 mutations were similar to the first virus including c. 241 C > T at 5'UTR, 3037 C > T in ORF1ab, NSP3, 14408 C > T in ORF1ab. NSP12, 18877 C > T inORF1ab, NSP14, 22191-3 DEL, 22444 C > T and 23403 A > G in Spike, 25563 G > T in NS3, 26735 C > T in Matrix and 28854 C > T in Nucleocapsid (Table 2). |