Mutation detail:
| Mutation site | L1421F |
| Virus | Human respiratory syncytial virus |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | L |
| Gene ID | 1494467 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | A |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | P28887.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | Yes |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Polymerase |
| Uniprot protein ID | P28887 |
| Protein length | 2165 amino acids |
| Protein description | The core polymerase in RSV is composed of two proteins, a 250 kDa polymerase subunit (L), which contains the RNA-dependent RNA polymerase, the polyribonucleotidyl transferase (PRNTase, capping), and the methyltransferase enzymatic domains essential for viral transcription and replication, and the 27 kDa phosphoprotein (P) accessory protein. . The L protein caps mRNA by a unique RNA-GDP polyribonucleotidyl transferase activity mapped to conserved region V (CRV). Methylation of the cap at the guanine-N-7 and ribose-2-O positions is catalyzed by a unique dual specificity methyltransferase activity that has been functionally mapped to region VI of the L protein. |
Literature information:
| Pubmed ID | 31495574 |
| Clinical information | No |
| Disease | - |
| Published year | 2019 |
| Journal | Cell |
| Title | Structure of the Respiratory Syncytial Virus Polymerase Complex |
| Author | Morgan S.A. Gilman,Cheng Liu,Amy Fung,Ishani Behera,Paul Jordan |
| Evidence | Viruses that escape inhibition by BI-D harbor mutations encoding one of three substitutions in residues surrounding the PRNTase active site (I1381S, L1421F, or E1269D). |