Mutation detail:
| Mutation site | 18928C>T |
| Virus | SARS-CoV-2 |
Mutation level  |
Nucleotide level |
| Gene/protein/region type | ORF1ab(3'-to-5' exonuclease) |
| Gene ID | 43740578 |
| Country | India |
| Mutation type |
- |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | - |
| Viral reference sequence | NC_045512.2 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF1ab polyprotein |
| Uniprot protein ID | P0DTC1 |
| Protein length | 7096 amino acids |
| Protein description | ORF1ab, the largest gene, contains overlapping open reading frames that encode polyproteins PP1ab and PP1a. The polyproteins are cleaved to yield 16 nonstructural proteins, NSP1-16. Production of the longer (PP1ab) or shorter protein (PP1a) depends on a -1 ribosomal frameshifting event. The proteins, based on similarity to other coronaviruses, include the papain-like proteinase protein (NSP3), 3C-like proteinase (NSP5), RNA-dependent RNA polymerase (NSP12, RdRp), helicase (NSP13, HEL), endoRNAse (NSP15), 2'-O-Ribose-Methyltransferase (NSP16) and other nonstructural proteins. SARS-CoV-2 nonstructural proteins are responsible for viral transcription, replication, proteolytic processing, suppression of host immune responses and suppression of host gene expression. The RNA-dependent RNA polymerase is a target of antiviral therapies. |
Literature information:
| Pubmed ID | 33683658 |
| Clinical information | No |
| Disease | - |
| Published year | 2021 |
| Journal | VIRUS GENES |
| Title | Isolation and genetic characterization of SARS-CoV-2 from Indian patients in a single family without H/O travel abroad |
| Author | Shubham Shrivastava, Harshad P. Patil, Suhas T. Mhaske, Sonali Palkar, Sanjay Lalwani |
| Evidence | The complete genome length of 8003 and 8004 viruses were 29,866 nucleotides (nt) with 5 untranslated region (UTR) of 265nt and 3 UTR of 188nt. These sequences were compared with reference sequence, NC045512 from Wuhan, China. At nucleotide level, IRSHA isolates were 99.96 identical to the reference sequence. The distribution of the 11 mutations recorded was C241T (5 UTR), C313T, C3037T, C5700A, C14408T, C18928T (ORF1ab), A23403G, G23593C (spike protein) and G28881A, G28882A, G28883C (nucleocapsid phosphoprotein) (Table1). Two mutations at positions, C313T and C3037T, were synonymous mutations with no change in amino acids, leucine (L) and phenylalanine (F). Eight mutations resulted in amino acid changes at positions 5700 (nsp3, A1812D), 14,408 (RdRp, P314L), 18,928 (3 to 5 exonuclease, exoN, P1821S), 23,403 (spike protein, D614G), and 23,593 (spike protein, Q677H). The mutations at G28881A, G28882A and G28883C resulted in amino acid changes, R9455K and G9456R in nucleocapsid (203 and 204) region. |