Mutation detail:
| Mutation site | R38A/K41A |
| Virus | Influenzavirus A H1N1 |
Mutation level  |
Amino acid Level |
| Gene/protein/region type | NS1 |
| Gene ID | 23308111 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | LC333189.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Nonstructural Protein 1 |
| Uniprot protein ID | C3W611 |
| Protein length | 219 amino acids |
| Protein description | The non-structural protein 1 (NS1) is a crucial influenza A virus immune regulator that antagonizes antiviral response. The NS1 protein counteracts the production of IFNs and the activities of IFN-induced proteins that restrict influenza virus replication. Furthermore, NS1 is a multifunctional regulatory viral protein that plays a critical role as a posttranscriptional regulatory factor in the life cycle of influenza virus; specifically, binding virion RNA. NS1 protein consists of 2 distinct functional domains connected by a flexible linker region (LR): an N-terminal RNA- binding domain (RBD, aa 1-73) and a C-terminal effector domain (ED, aa 74-207). The nucleotide sequence (NLS) (aa 35-41) overlaps the sequences needed for dsRNA binding. |
Literature information:
| Pubmed ID | 17442719 |
| Clinical information | No |
| Disease | - |
| Published year | 2007 |
| Journal | JOURNAL OF VIROLOGY |
| Title | Multiple Anti-Interferon Actions of the Influenza A Virus NS1 Protein |
| Author | Georg Kochs,Adolfo García-Sastre,Luis Martínez-Sobrido |
| Evidence | A recombinant virus with inactivating mutations in NS1 (R38A/K41A), called WSN(Mut), which was shown to be a strong IFN inducer (15), was used as a positive control. WSN(Mut) strongly induced IFN-expression, whereas infection with the viruses carrying the functional NS gene segments, i.e., WSN(PR) and WSN(Tx), showed only weak induction, as assessed by RT-PCR analysis (Fig.1A). |