Mutation detail:
| Mutation site | K543A/R544A |
| Virus | SARS-CoV |
Mutation level  |
Amino acid level |
| Gene/protein/region type | S Protein |
| Gene ID | 1489668 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | AY291315.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | spike glycoprotein |
| Uniprot protein ID | P59594 |
| Protein length | 1255 amino acids |
| Protein description | The spike protein of SARS coronaviruses is a protein composed of three polypeptide chains and it contains two domains, S1 and S2. The S1 domain binds the host cell receptors, while the S2 domain is responsible for the fusion of the virus with the host cell membrane. Between S1 and S2, there is a hinge region which is targeted by the host cell proteases. |
Literature information:
| Pubmed ID | 28636671 |
| Clinical information | No |
| Disease | - |
| Published year | 2017 |
| Journal | PLoS One |
| Title | Different residues in the SARS-CoV spike protein determine cleavage and activation by the host cell protease TMPRSS2 |
| Author | Lennart Michel Reinke, Martin Spiegel, Teresa Plegge, Anika Hartleib, Inga Nehlmeier |
| Evidence | Immunofluorescence of transfected COS-7 cells coexpressing SARS S wt and mutants jointly with YFP targeted to the plasma membrane revealed that SARS S wt and mutants R667A, T678S and R797N were trafficked to the cell surface, although intracellular levels |