Mutation detail:
| Mutation site | N15A |
| Virus | SARS-CoV |
Mutation level  |
Amino acid level |
| Gene/protein/region type | E Protein |
| Gene ID | 1489671 |
| Country | - |
| Mutation type |
nonsynonymous mutation |
| Genotype/subtype/clade | - |
| Sample |
cell line |
| Variants | - |
| Viral reference sequence | AY278741.1 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
decrease |
| Pathogenicity mechanism | Mutations N15A and V25F located in the transmembrane domain prevented the ion conductivity. |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | Small Envelope Protein |
| Uniprot protein ID | P59637 |
| Protein length | 76 amino acids |
| Protein description | The E protein of SARS coronavirus contains three domains, N- terminal domain, transmmebrane domain, and C-terminal domain. The C- terminal localizes in the Endoplasmic Reticulum and Golgi complex to participate in the assembling and budding of the virions inside the host cells. The functions of the E protein include the assembly of the virion envelope; suppression of the host cells stress responses which is important for the downregulation of immune response and promotion of pathogenesis. |
Literature information:
| Pubmed ID | 22832120 |
| Clinical information | No |
| Disease | - |
| Published year | 2012 |
| Journal | Virology |
| Title | Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids |
| Author | Carmina Verdia-Baguena, Jose L. Nieto-Torres, Antonio Alcaraz, Marta L. DeDiego, Jaume Torres |
| Evidence | A total of nine peptides derived from SARS-CoV E protein were synthesized, one of them (wt) representing the wild type protein transmembrane domain and its flanking polar amino acids, and eight mutant peptides including different residue substitutions. On |