AVM

Protein name	ORF1ab polyprotein
Uniprot protein ID	P0C6X7
Protein length	7073 amino acids
Protein description	The Orf1ab polyprotein contains the Orf1a polyprotein and 16 non-structural proteins. The Orf1ab polyprotein and its cleavage products carry out a wide range of activities associated with the viral replication, including ATP binding and breaking down of ADP and phosphate, cysteine-directed endopeptidase, leading to the formation of nonstructural proteins, such as exonuclease that produces ribose- 5-phosphate, methyltransferase for the synthesis of new nucleotides, RNA polymerase for the viral replication, RNA helicase for the removal of super twisting, as well as the regulation of the transcription through binding of zinc. However, the functions of the Orf1ab are the collective functions of the non-structural proteins encoded by the gene of Orf1ab

Pubmed ID	21390281
Clinical information	No
Disease	-
Published year	2011
Journal	PLoS Computational Biology
Title	Dynamically-Driven Inactivation of the Catalytic Machinery of the SARS 3C-Like Protease by the N214A Mutation on the Extra Domain
Author	Jiahai Shi, Nanyu Han, Liangzhong Lim, Shixiong Lua, J. Sivaraman
Evidence	Therefore, the N214A mutation appears to trigger the dramatic change of the enzyme dynamics in the context of the dimeric form which ultimately inactivates the catalytic machinery.