Mutation detail:
| Mutation site | 27367C>T |
| Virus | SARS-CoV-2 |
Mutation level  |
Nucleotide level |
| Gene/protein/region type | ORF6 |
| Gene ID | 43740572 |
| Country | Italy |
| Mutation type |
- |
| Genotype/subtype/clade | - |
| Sample |
Human |
| Variants | Alpha |
| Viral reference sequence | NC_045512.2 |
| Drug/antibody/vaccine | - |
| Transmissibility |
- |
| Transmission mechanism | - |
| Pathogenicity |
- |
| Pathogenicity mechanism | - |
| Immune escape mutation | - |
| Immune escape mechanism | - |
| RT-PCR primers probes | - |
Protein detail:
| Protein name | ORF6 protein |
| Uniprot protein ID | P0DTC6 |
| Protein length | 61 amino acids |
| Protein description | ORF6 encodes a viral accessory protein. Based on its similarity to other coronavirus proteins, ORF6 protein is thought to play a role in viral pathogenesis.The functions of orf6 are as follows: 1) it binds the nsp8 in the replication\transcription complex which reflects its importance for the viral replication, 2) it increases the infection titer, 3) it contributes to the evasion of the virus to the host immune system through inhibition of the interferon induction and signaling pathway; it inhibits the Signal Transduction and Activator of Transcription (STAT 1), and 4) it participates in the formation of the DMV in the host cells which helps in protein trafficking to ensure the assembling of the virus. |
Literature information:
| Pubmed ID | 33525998 |
| Clinical information | No |
| Disease | - |
| Published year | 2021 |
| Journal | Emerging Microbes & Infections |
| Title | Isolation of SARS-CoV-2 strains carrying a nucleotide mutation, leading to a stop codon in the ORF 6 protein |
| Author | Serena Delbue, Sarah D Alessandro,Lucia Signorini,Maria Dolci,Elena Pariani |
| Evidence | The complete genome sequences show that the two isolates belong to the B1.1 lineage, but contain a nucleotide mutation in the ORF6, leading to a stop codon and to the deletion of 6 amino acids in the C terminus |